Extraction of chitin from edible crab shells of Callinectes sapidus and comparison with market purchased chitin

Abstract Chitin and its derived products have immense economic value due to their vital role in various biological activities as well biomedical industrial application. Insects, microorganism crustaceans are the main supply of chitin but shell like shrimp, krill, lobsters crabs commercial sources. content an individual varies depending on structures possessing polymer species. In this study edible crabs’ shells (Callinectes sapidus) were demineralized deproteinized resulting 13.8% (dry weight) recovery from wastes. FTIR XRD analyses experimental crude purified chitins revealed that both much comparable commercially purchased controls. The acid pretreatment ceded 54g colloidal resulted 1080% chitin. was exploited for isolation eighty five chitinolytic bacterial isolates different Zone clearance displayed by thirty (41.17%) succeeding growth at pH 7 agar medium. Maximum activity i.e. 301.55 U/ml exhibited isolate JF70 when cultivated extracted containing carbon nitrogen. showed wastes blue can be utilized extraction bacteria used degrade waste, resolve environmental pollution purpose.